The insert contains internal sites for AvaII, AluI, BamHI and SmaI. Restriction digests of the clone give the following sizes (kb): HindIII--4.4, 0.4; PstI--4.8; EcoRI--4.8. A full-length (359 bp) cDNA clone with HaeIII ends modified by HindIII linkers. It is non-infectious. |