ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BglI--3.0, 1.15; BglII--4.1; EcoRI--2.7, 1.5; PvuII--2.3, 1.4, 0.4; SstI--3.8, 0.35.
The insert contains the following restriction sites (nt position according to the published sequence): PstI--563, 610, 939, 1063; BglII--610; SacI--1109; PvuII--1214.
Contains 5' untranslated sequences and the 5' end of the coding region which begins at nt 398 of the published sequence.
References
Strehler EE, et al. Peptide sequence analysis and molecular cloning reveal two calcium pump isoforms in the human erythrocyte membrane. J. Biol. Chem. 265: 2835-2842, 1990. PubMed: 2137451