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Size (kb): 5.3800001144409180

DESCRIPTION OF VECTOR:
Intact vector size: 5.380
Type of vector: phagemid
Cloning sites: SpeI BamHI SmaI EcoRI EcoRV ClaI SalI XhoI
Polylinker sites: SpeI BamHI SmaI PstI EcoRI EcoRV HindIII ClaI SalI Xho
Construction: pRS416, CUP1 promoter
Host range: Saccharomyces cerevisiaeCandida robusta; Escherichia coli
Features (with orientation and position when available):
marker(s): URA3, ->
replicon: f1, <-
terminator: CYC1, <-
MCS: XhoI...SpeI, ->
promoter for expression: CUP1, ->
replicon: pMB1
marker(s): ampR, <-
centromere: CEN6
replicon: ARSH4

Vector: pCu416CUP1 (phagemid)

Promoters: Promoter for expression CUP1

Construction: pRS416, CUP1 promoter

Marker(s):URA3,ampR

Construct size (kb): 5.380000114440918

Features: marker(s): URA3
marker(s): ampR
promoter for expression: CUP1
replicon: ARSH4
replicon: f1
replicon: pMB1
MCS: XhoI...SpeI
terminator: CYC1
centromere: CEN6

cloning vector

Restiction digests of the vector give the following sizes (kb): SacI--5.4; SacI/SpeI--5.4 (has lost SpeI site).

Gene CUP1 is the most potent for copper induction. The CUP1 promoter can be used to creat a series of versatile vectors to allow its utilization to control the expression of heterologous genes in S.cerevisiae.

component of:ATCC 87778