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pNKY150 [Tn10 URA Blaster]
pNKY150 [Tn10 URA Blaster]
規(guī)格:
貨期:
編號(hào):B236847
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱(chēng) pNKY150 [Tn10 URA Blaster]
商品貨號(hào) B236847
Designations pNKY150 [Tn10 URA Blaster]
Species Saccharomyces cerevisiae Meyen ex E.C. Hansen
Depositors N Kleckner
Applications
A plasmid containing an excisable URA3 cartridge for gene disruption that permits repeated use of URA3 selection in constructing multiply disrupted yeast strains.
Vector
Construct size (kb): 8.5
Insert
DNA: genomic
Insert lengths(kb): 4.599999904632568
Gene product: uridine monophosphate synthetase( UMP synthase, orotate phosphoribosyltransferase, orotidine 5'-phosphate decarboxylase, orotate phosphoribosyltransferase 1) [URA3]
Insert Size (kb) 4.600
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Restriction digests of the clone give the following sizes (kb): BamHI/BglII--5.0, 4.2; BamHI--9.2; EcoRI--9.2; PstI--6.6, 2.6; XbaI--uncut.
Used by inserting the excised cassette into a cloned target gene, isolating a restriction fragment containing the cassette with flanking target gene sequences, transforming Ura3- strains, and selecting for stable integrants.
Loss of the cassette (and one of the flanking IS10 repeats) by recombination can be selected by 5-fluoro-orotic acid resistance.
A plasmid containing an excisable URA3 cartridge for gene disruption that permits repeated use of URA3 selection in constructing multiply disrupted yeast strains.
The BglII/BamHI cassette contains URA3 flanked by 1.5 kb direct repeats from insertion sequence IS10.
Classification Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae
References

Alani E, et al. A method for gene disruption that allows repeated use of URA3 selection in the construction of multiply disrupted yeast strains. Genetics 116: 541-545, 1987. PubMed: 3305158

Riles L, et al. Physical maps of the six smallest chromosomes of Saccharomyces cerevisiae at a resolution of 2.6-kilobase pairs. Genetics 134: 81-150, 1993. PubMed: 8514151

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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