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phAT4.8 [phAT4.4]
phAT4.8 [phAT4.4]
規(guī)格:
貨期:
編號(hào):B231437
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 phAT4.8 [phAT4.4]
商品貨號(hào) B231437
Designations phAT4.8 [phAT4.4]
GenBank Number

K02212

Species Homo sapiens, human
Depositors SL Woo
Vector
Construct size (kb): 11.80000019073486
Insert
DNA: genomic
Insert lengths(kb): 4.800000190734863
Tissue: liver
Gene product: alpha-1-antitrypsin (protease inhibitor) [PI]
Alleles: G3, A1, A2, B1, B2, D2, G1, G2, J2, N1, N2, C1, C2, D1, J1
Insert Size (kb) 4.800
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--7.0, 4.8; BamHI--10.6, 1.2; HindIII--4.2, 3.6, 3.5, 0.7; PvuII--6.5, 2.7, 1.75, 0.85; PstI--3.4, 3.4, 1.3, 0.8, 0.5.
The insert contains 2 BamHI sites (upstream of exon 1) and 2 HindIII sites (in intron 1).
IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.
The insert is the genomic EcoRI fragment containing 5' flanking sequences, exon 1, and part of intron 1.
References

Long GL, et al. Complete sequence of the cDNA for human alpha1-antitrypsin and the gene for the S variant. Biochemistry 23: 4828-4837, 1984. PubMed: 6093867

Sifers RN, et al. A frameshift mutation results in a truncated alpha1-antitrypsin that is retained within the rough endoplasmic reticulum. J. Biol. Chem. 263: 7330-7335, 1988. PubMed: 3259232

Cox DW, et al. DNA restriction fragments associated with alpha1-antitrypsin indicate a single origin for deficiency allele PI Z. Nature 316: 79-81, 1985. PubMed: 2989709

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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