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HFBEK45 [XPB]
HFBEK45 [XPB]
規(guī)格:
貨期:
編號(hào):B231086
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 HFBEK45 [XPB]
商品貨號(hào) B231086
Designations HFBEK45 [XPB]
GenBank Number

T07528

Species Homo sapiens, human
Vector
Construct size (kb): 4.599999904632568
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ', <-
primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, <-
promoter: lac, <-
primer site: M13 reverse, <-
replicon: pMB1
marker(s): ampR, <-
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: XBP1
Other gene symbols: XBP2
Genomic copy number: unique
Gene name: X-box binding protein 1
Contains complete coding sequence?: N
Chromosome: 22; Localization: 22; 22, cR
Tissue: fetal brain, female, 17-18 wk (Stratagene 936206)
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 1.57
Cross references: DNA Seq. Acc.: M31627; EST: EST05418
Nucleotides 1-343 of the insert correspond to
nucleotides 313-655 of M31627 with 99% identity.
DNA Seq. Acc.: T07528; EST: EST05418; Primer: M13-21; THC
id: THC122879
Insert lengths(kb): 1.570000052452087
Tissue: fetal brain, female, 17-18 wk (Stratagene 936206)
Gene product: X-box binding protein 1 [XBP1]
Insert Size (kb) 1.570
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--3.1, 0.63, 0.56, 0.38.
The insert contains multiple EcoRI fragments. It is important to note these may have been derived from different cDNAs.
Based on the alignment with GenBank M31627 (1818 nt, CDS 13-795) and the insert size, this clone appears to contain a portion of the coding sequence.
References

Adams MD, et al. 3400 expressed sequence tags identify diversity of transcripts from human brain. Nat. Genet. 4: 256-267, 1993. PubMed: 8358434

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