Restriction digests of the clone give the following sizes (kb): BamHI--2.75, 0.45; BglI--2.1, 1.15; EcoRI--2.7, 0.5; PstI--2.8, 0.35; PvuII--2.4, 0.8. The insert includes the following restriction sites (nt from the 5' end): BamHI--37; PstI--152. Contains the complete coding sequence (nt 73-459). Detects human mRNA of the following approximate sizes (kb): 0.8, 1.4, 2.0. |