產(chǎn)品名稱 |
Enterovirus D68 |
商品貨號 |
B217707 |
Classification |
Picornaviridae, Enterovirus, Enterovirus D |
Agent |
Enterovirus D68 |
Strain |
US/KY/14-18953 |
Biosafety Level |
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
Product Format |
frozen 1 mL per vial |
Storage Conditions |
-70°C or colder |
Images |
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Effect on Host |
CPE, refractile rounding and sloughing |
Recommended Host |
RD cells (ATCC® CCL-136™) |
Growth Conditions |
Temperature: 33°C
Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 80-95% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.01-0.001). Adsorb 1-2 hours at 33°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium.
Incubation: 2-3 days |
Name of Depositor |
Centers for Disease Control and Prevention |
Source |
Nasopharyngeal swab from patient with respiratory illness, Kentucky, September 2014 |
References |
Brown BA, et al. Seven Strains of Enterovirus D68 Detected in the United States during the 2014 Severe Respiratory Disease Outbreak. Genome Announc 2(6): e01201-14, 2014. PubMed: 25414503
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