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HUVEC/TERT 2
HUVEC/TERT 2
規(guī)格:
貨期:
編號:B216124
品牌:Mingzhoubio

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產(chǎn)品名稱 HUVEC/TERT 2
商品貨號 B216124
Organism Homo sapiens, human
Tissue Umbilical vascular endothelium
Cell Type Endothelial cell immortalized with hTERT
Product Format frozen 1 mL
Morphology Cobblestone appearance with large dark nuclei
Culture Properties adherent
Biosafety Level 2 [These primary cells are not known to harbor an agent recognized to cause disease in healthy adult humans. Handle as a potentially biohazardous material under at least Biosafety Level 1 containment. Cells derived from primate lymphoid tissue may fall under the regulations of 29 CFR 1910.1030 Bloodborne Pathogens. 

ATCC recommends that appropriate safety procedures be used when handling all primary cells and cell lines, especially those derived from human or other primate material. Detailed discussions of laboratory safety procedures are provided in Laboratory Safety: Principles and Practice, 2nd ed. (ASM Press, Washington, DC) (Fleming et al., 1995) and Caputo, J.L. Biosafety procedures in cell culture. (1988) J. Tissue Culture Methods 11:223. Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the following publication: Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS Publication No. (CDC) 93-8395. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online at http://www.cdc.gov/biosafety/publications/bmbl5/index.htm.] 

Human Material Precaution 

All tissues used for isolation are obtained under informed consent and conform to HIPAA standards to protect the privacy of the donor’s personal health information. It is best to use caution when handling any human cells. We recommend that all human cells be accorded the same level of biosafety consideration as cells known to carry HIV. With infectious virus assays or viral antigen assays, even a negative test result may leave open the possible existence of a latent viral genome.


Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease Normal
Age Newborn
Gender female
Ethnicity Caucasian
Applications Immune response, wound healing, cellular response to viral or bacterial infection, oxidative stress, angiogenesis, arteriolosclerosis, drug screening, tubule formation
Shipping Information Frozen 1 mL
Storage Conditions -130°C
Karyotype 46,XX,add(14)(p10)
Images Cell Micrograph of HUVEC/TERT2 cells, CRL-4053
Derivation Umbilical vascular endothelium transduced with hTERT
Antigen Expression CD31 >75% positive
Receptor Expression LDL uptake >75%positive
Complete Growth Medium The base medium for this cell line is Vascular Cell Basal Medium (ATCC® PCS-100-030), supplemented with Vascular Endothelial Cell Growth Kit-VEGF (ATCC® PCS-100-041). Alternatively, the Vascular Cell Basal Medium can be supplemented with Endothelial Cell Growth Kit-BBE (ATCC® PCS-100-040).
Subculturing Every 3-4 days at 8000 cells/cm2 Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation solutions for culture vessels of other sizes.

  1. Remove and discard spent medium.
  2. Briefly rinse with 1X PBS (ATCC 30-2200), 1 mL/25 cm2 and discard rinse solution.
  3. Add trypsin for primary cells (ATCC PCS-999-003), 1 mL/25 cm2.  Place at 37oC for 3-4 minutes, until 90% of the cells have detached. 
  4. Rapflask gently to ensure cells are detached. Add 2% FBS in D-PBS, 1 mL/25 cm2 to neutralize trypsin.
  5. Centrifuge cells at 150 x g for 5 min at room temperature.
  6. Remove supernatant. Resuspend pellet in 5.0 to 8.0 mL Complete Growth Medium.
  7. Count cells, and seed 8.0 x 103 to 10.0 x 103 viable cells/cm2 to new culture vessels.
Medium Renewal: Every 2-3 days.
Cells per Vial 1.2 x 106 cells/vial
Volume 1 mL
STR Profile Amelogenin: X
CSF1PO: 12
D5S818: 15, 16
D7S820: 9, 11
D13S317: 11, 12
D16S539: 9, 13
TH01: 6, 9
TPOX: 8, 9
vWA: 16, 19

CO1 Species: Human

Sterility Tests Bacteria and Yeast: Negative
Mycoplasma: Negative
Viability >70% when thawed from cryopreservation
Population Doubling Capacity At least 25 PDL
Population Doubling Time 2.5 days
Name of Depositor Evercyte
Year of Origin 2011
梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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