| 產(chǎn)品名稱 | F98 EGFR |
|---|---|
| 商品貨號 | B215043 |
| Organism | Rattus norvegicus, rat |
| Tissue | brain |
| Product Format | frozen |
| Morphology | glial |
| Culture Properties | adherent |
| Biosafety Level | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | undifferentiated malignant glioma |
| Age | fetus, 20 days gestation |
| Applications | This cell line has been used for in vivo studies of molecular targeting of EGFR in synegeneic Fischer Rats using either the ligand EGF Ref
|
| Storage Conditions | liquid nitrogen vapor phase |
| Images |  |
| Derivation | The F98EGFR was produced by transfecting parental F98 rat glioma cells (ATCC CRL-2397) with an expression vector containing human, wildtype EGFR cDNA. |
| Receptor Expression | Epidermal Growth Factor Receptor (EGFR), expressed (verified at ATCC) |
| Tumorigenic | YES |
| Effects | tumorigenic in syngeneic Fischer rats |
| Comments | F98EGFR cells express approximately 5 x 105 non-functional EGFR sites per cell compared with an undetectable number of EGFR sites on F98 parental cells. |
| Complete Growth Medium | The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002.
To make the complete growth medium, add the following components to the base medium:
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| Subculturing | Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Note: If cells are cultured continuously, add 0.6 mg/mL G-418 to the complete growth medium for a passage about once a month. Subcultivation ratio: A subcultivation ratio of 1:6 to 1:10 is recommended. Medium renewal: Every 2 to 3 days.
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| Cryopreservation | Freeze medium: complete growth medium, 95%; DMSO, 5% liquid nitrogen vapor phase |
| Culture Conditions | Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| Name of Depositor | RF Barth |
| Year of Origin | May 1999 |
| References | Yang W, et al. Convection enhanced delivery of boronated epidermal growth factor for molecular targeting of EGFR positive gliomas. Cancer Res. 62: 6552-6558, 2002. PubMed: 12438250 Barth RF, et al. Molecular targeting of the epidermal growth factor receptor for neutron capture therapy of gliomas. Cancer Res. 62(11): 3159-3166, 2002. PubMed: 12036929 Wu G, et al. Targeted delivery of methotrexate to epidermal growth factor receptor-positive brain tumors by means of cetuximab (IMC-C225) dendrimer bioconjugates. Mol. Cancer Ther. 5(1): 52-59, 2006. PubMed: 16432162. Wu G, et al. Molecular targeting and treatment of an epidermal growth factor receptor-positive glioma using boronated cetuximab. Clin. Cancer Res. 13(4): 1260-1268, 2007. PubMed: 17317838 Yang W, et al. Molecular targeting and treatment of composite EGFR and EGFRvIII-positive gliomas using boronated monoclonal antibodies. Clin. Cancer Res. 14(3): 883-891, 2008. PubMed: 18245552 |
| 梅經(jīng)理 | 17280875617 | 1438578920 |
| 胡經(jīng)理 | 13345964880 | 2438244627 |
| 周經(jīng)理 | 17757487661 | 1296385441 |
| 于經(jīng)理 | 18067160830 | 2088210172 |
| 沈經(jīng)理 | 19548299266 | 2662369050 |
| 李經(jīng)理 | 13626845108 | 972239479 |
Barth RF, et al. Molecular targeting of the epidermal growth factor receptor for neutron capture therapy of gliomas. Cancer Res. 62(11): 3159-3166, 2002. PubMed: 12036929