Tetramitis thorntoni (Singh) Brown and De Jonckheere
商品貨號(hào)
B213413
Deposited As
Naegleria thorntoni (Singh) Page
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation
farmyard soil, England, 1950 (?)
Product Format
freeze-dried
Type Strain
no
Comments
Zymodeme Th-1
Maximum temperature tolerance 37C
Circular ribosomal RNA genes
Transfer of species back to Didasculus based upon SSUrDNA sequence
review
Medium
ATCC® Medium 997: Fresh water ameba medium
Growth Conditions
Temperature: 25.0°C
Duration: grown with Escherichia coli ATCC 11775
Protocol: ATCCNO: 30133 SPEC: This strain is shipped as a dried preparation. Aseptically add 1 ml of sterile distilled water to the inner vial, remove the filter paper pellet with a pair of forceps, and place it in the center of an agar plate of ATCC medium 711 or 997. Add the liquid remaining in the vial to the plate and spread it evenly over the surface of the plate. Incubate the plate at 25C. Trophozoites (amebae) should be evident within 2-3 days.
Subcultivation
Protocol: ATCCNO: 30133 SPEC: This strain is shipped as a dried preparation. Aseptically add 1 ml of sterile distilled water to the inner vial, remove the filter paper pellet with a pair of forceps, and place it in the center of an agar plate of ATCC medium 711 or 997. Add the liquid remaining in the vial to the plate and spread it evenly over the surface of the plate. Incubate the plate at 25C. Trophozoites (amebae) should be evident within 2-3 days.
Clark CG, Cross GA. Circular ribosomal RNA genes are a general feature of schizopyrenid amoebae. J. Protozool. 35: 326-329, 1988. PubMed: 2840492
De Jonckheere JF, et al. On the identity of the amoeboflagellates Didascalus thorntoni and Adelphamoeba galeacystis. J. Eukaryot. Microbiol. 44: 52-54, 1997. PubMed: 9172832
John DTOpportunistically pathogenic free-living amebaeIn: John DTParasitic protozoa2nd ed.3San DiegoAcademic Presspp. 143-246, 1993
Adams M, et al. A genetic approach to species criteria in the amoeba genus Naegleria using allozyme electrophoresis. Int. J. Parasitol. 19: 823-834, 1989. PubMed: 2635158
