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Size (kb): 6.6370000839233400

DESCRIPTION OF VECTOR:
Intact vector size: 6.637
Type of vector: plasmid
Cloning sites: XhoI SalI ClaI HindIII EcoRI SmaI BamHI SpeI
Polylinker sites: XhoI SalI ClaI HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI
Other unique sites: SacI
Construction: pRS426 (ATCC 77107)
Host range: Saccharomyces cerevisiaeCandida robusta; Escherichia coli
Features (with orientation and position when available):
Marker(s): URA3, ->, 200-1300
Terminator: CYC1, ->, 2004-2264
MCS: XhoI...XbaI, ->, 2265-2339
Promoter for expression: GPD, <-, 2339-3019
Marker(s): ampR, <-, 4138-5068
Replicon: 2 micron, ->, 5167-6507

Vector: p426 GPD (plasmid)

Promoters: Promoter for expression GPD

Construction: pRS426 (ATCC 77107)

Marker(s): URA3,ampR

Construct size (kb): 6.63700008392334

Features: marker(s): URA3
Marker(s): ampR
Promoter for expression: GPD
Replicon: 2 micron
MCS: XhoI...XbaI
Terminator: CYC1

YE-type (episomal) shuttle vector

YX-type (expression) shuttle vector

Restriction digests of the clone give the following sizes (kb): SacI/XbaI--3.2, 2.8, 0.7; EcoRI--6.6; BamHI--6.6.

High copy number shuttle expression vector.

Temperature: 37°C

component of:ATCC 87669