日韩精品人妻一区二区中文,五月婷婷综合在线视频,A久久精品国产精品亚洲,亚州中文精品有码视频在线,精品1区2区3区产品乱码,色哟哟免费观看视频入口,婷婷射精AV这里只有精品,2024日产乱码国产,国产精品三级一区二区,艳妇乳肉豪妇荡乳AV

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當(dāng)前位置: 首頁 > ATCC代理 > EL4-Luc2
▍最近瀏覽歷史
▍聯(lián)系我們
  • 0574-87157013
  • mingzhoubio@163.com
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號
  • 創(chuàng)e慧谷42號樓B幢401室
EL4-Luc2
EL4-Luc2
規(guī)格:
貨期:
編號:B161693
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 EL4-Luc2
商品貨號 B161693
Organism Mus musculus, mouse
Tissue blood
Product Format frozen 1.0 mL
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease lymphoma
Strain C57BL/6N
Applications Excellent signal/background ratio and stable luciferase expression make this cell line ideal for in vivo bioluminescence imaging of xenograft animal model to study human cancer and monitor activity of anti-cancer drugs. It also can be used in cell-based assays for cancer research.
Comments This luciferase expressing cell line was derived from parental line (ATCC TIB-39) by transduction with lentiviral vector encoding firefly luciferase gene (luc2) under control of EF-1 alpha promoter. This cell line was established through single cell cloning, and the cells constitutively express high levels of enzymatically active luciferase protein, which can be detected via in vitro and in vivo bioluminescence assays. The cells should be maintained in blasticidin (4 μg/mL) containing medium in routine cell culture. It is recommended to remove blasticidin prior to and during the experiment procedure when the cells are injected into animals in vivo, or co-cultured with other cell types in vitro.
Complete Growth Medium

The base medium for this cell line is ATCC-formulated Modified Eagle's Medium (DMEM, ATCC 30-2001). To make the complete growth medium, add the following components to the base medium:

  • Horse serum (FBS; ATCC 30-2040) to a final concentration of 10%
  • Blasticidin to a final concentration of 4 μg/mL
Subculturing
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 x 105 to 4.0 x 10 5 cells/mL and maintain between 1 x 105 and 1 x 106 cells/mL.

Medium Renewal: every 2 to 3 days (depending on cell density).
Cryopreservation Complete growth medium supplemented with 5% (v/v) DMSO
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
Cells per Vial ≥ 1.0 x 106 cells
Volume 1.0 mL
Sterility Tests Bacteria and yeast: No growth
Mycoplasma: No growth
Functional Tests Luciferase activity: signal to noise ≥ 1,000 RLUs
In Vitro Luminesence: 20,000 photons/cell/sec, subject to imaging and culturing conditions
Population Doubling Time approximately 19 hrs
Name of Depositor ATCC
Year of Origin 2018
References

Zinn KR, et al. Noninvasive bioluminescence imaging in small animals. ILARJ 49: 103-115, 2008. PubMed: 18172337

Dothager RS, et al. Advances in bioluminescence imaging of live animal models. Curr Opin Biotechnol 20: 45-53, 2009. PubMed: 19233638

Ralph P. Retention of lymphocyte characteristics by myelomas and theta+-lymphomas: sensitivity to cortisol and phytohemagglutinin. J. Immunol. 110: 1470-1475, 1973. PubMed: 4541304

Old LJ, et al. The G (Gross) leukemia antigen. Cancer Res. 25: 813-819, 1965. PubMed: 4284252

Gorer PA. Studies in antibody response of mice to tumour inoculation. Br. J. Cancer 4: 372-379, 1950. PubMed: 14801344

Herberman RB. Serological analysis of cell surface antigens of tumors induced by murine leukemia virus. J. Natl. Cancer Inst. 48: 265-271, 1972. PubMed: 4119883

Ralph P, Nakoinz I. Inhibitory effects of lectins and lymphocyte mitogens on murine lymphomas and myelomas. J. Natl. Cancer Inst. 51: 883-890, 1973. PubMed: 4542714

Shevach EM, et al. Immunoglobulin and theta-bearing murine leukemias and lymphomas. J. Immunol. 108: 1146-1151, 1972. PubMed: 4112916

Aparicio CL, et al. Correction for label leakage in fluorimetric assays of cell adhesion. BioTechniques 23: 1056-1060, 1997. PubMed: 9421636

Cuthbert JA, Lipsky PE. Regulation of proliferation and Ras localization in transformed cells by products of mevalonate metabolism. Cancer Res. 57: 3498-3504, 1997. PubMed: 9270019

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
莱芜市| 晋江市| 工布江达县| 楚雄市| 沙坪坝区| 高碑店市| 嵊泗县| 浙江省| 绩溪县| 九龙县| 灵宝市| 张家川| 吐鲁番市| 东宁县| 富阳市| 利辛县| 红安县| 都匀市| 河间市| 麻江县| 朝阳区| 安康市| 渑池县| 灵石县| 苗栗市| 霍邱县| 五台县| 敦化市| 新田县| 三江| 峨边| 洛川县| 廉江市| 华亭县| 鱼台县| 鹤壁市| 伊宁市| 玛曲县| 安图县| 贞丰县| 年辖：市辖区|